One Minute Pitch
RNAscope® ISH delivers single-molecule sensitivity and specificity. The multiplexing capability enables detection of multiple markers at once, enabling exquisite characterization of cell populations, co-localization/ co-expression studies and also sub-cellular localization of mRNA within the nervous system. Combine with Immunohistochemistry/ IF. Same-day results and automated assays mean you can get the data you seek quickly, easily, and all backed by our performance guarantee. |
Key Selling Point
Multiplex capability and dual ISH/IHC |
Overview
No Antibody? Detect GPCRs, Ion Channels, lncRNA
Validate RNA-Seq Signatures in single cells
Visualize cellular activity in cell type specific manner
Plasticity markers, Secreted factors, Co-localization
Characterize specific genetic variations
Alternative splicing, circRNA
Qualifying Questions
Do you want to explore molecular mechanisms of CNS/PNS development and neurological disorders like Alzheimer, Parkinson, Addictions, Pain, Depression...? |
Do you need to visualize biomarkers co-localization & expression in specific cellular sub-types? |
Are you looking for single cell resolution or need to distinguish expression of biomarkers between various nervous system cell types? |
Would you like to visualize sub cellular RNA/protein localization, axon/dendrites RNA transport? |
Are you investigating expression of splice variants (~90% of genes in the CNS have splice variants involved in specific diseases)? |
Do you need an assay for a non-commun target like for circRNA? |
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Pain Points and Our Solutions
Immunohistochemistry
Pain Point: Lack of antibody/specificity/secreted proteins/ lncRNA
Our Solution: Detection of any gene/ species/ tissue with highest specificity, new probe in 2 weeks, multiplex – up to 4 RNAs + 2 IHC targets. Co-expression studies with cell type markers, sub-cellular localization possible, detection of GPCRs/ Ion Channels |
Conventional ISH
Paint Point: Considered as complicated, long assay, dual ISH/IHC possible but really tricky
Our Solution: One day assay (not V2), easy-to-use, stable assay, great tech support (new user program), probe in depended optimization |
RNA-Seq/ RT-PCR
Pain Point: Give up tissue context, quantity of RNA required for qPCR is higher
Our Solution: Morphological context with single-cell specificity |
Recommended Products
RNAscope® Fluorescent Multiplex (320850) for fresh & fixed frozen
C1/C3: stronger channel (low expressor), C2: slightly weaker channel
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RNAscope® Multiplex Fluorescent v2 (323110) up to 4 targets, but ideal for FFPE, 4-plex: They need 4-Plex Ancillary Kit (323120) |
RNAscope® 2.5 HD Duplex Reagent Kit (322430) |
BaseScope for splice variants, circRNA |
Our Limitations
Multiplexing= With V2 6 Opal fluorophores available
(Theoretically 4x RNA, 2x protein as we only have 4 channels) |
ISH/IF= We can not guarantee results as depending on the antibody. |
Most Published Targets:
Gad1, Drd2, Drd1, VGluT2, Gad2, Th, Sst, EGFP, Shh, Vgat |
Long non-coding RNA in Neuroscience - examples
Alzheimer’s disease (BACE1-AS, SORL1, lncRNA 51A…)
Schizophrenia (Gomafu, DISC2…)
Autism spectrum disorder (MSNP1AS)
Parkinson’s disease (AL049437, AK021630...)
Huntington’s disease (Neat1) |
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