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Template for biochemical pathways Cheat Sheet by

A template that came handy for me to study Biochemistry: it requeires you to swicht from one format to another format. The process of analysing, then reconstructing is a good way to study anything.
biology     template     study     biochemistry     tool     pathway     metabolic

How the template works

The template for studying bioche­mical pathways works in real, physical paper: you get to use a pencil, rubber, and a ruler, and start sketching the pathway/s you need to study in the format provided here. When you analyze what you have and rebuild it in a different format, you're learning it.

Interp­ret­ation of the format provided

This is just a template for studying metabolic pathways, which uses the Krebs cycle to illustrate itself as an example. It uses alternate rows, as well as columns of input and columns of output. In the colored background rows we have the product of the previous reaction ( which is the reactive of the following reaction), next to the elements that are joined in the following reaction (INPUT), or those that are derived from the previous reaction (OUTPUT). The white rows contain the enzyme catalysing each reaction (with a number). These rows leave some space to the right to extra inform­ation, such as optimal conditions (pH, temper­ature, pressure ...), cofactors, the alpha of a reversible reaction (α), or whatever we want to study. As most bioche­mical reactions are revers­ible, those that are irreve­rsible (in this case: 1, 3 and 4) are being put between brackets, because they are decisive.

Krebs Cycle - Template Example

Produc­t/R­eactive
INPUT
OUTPUT
Step
Enzyme
Extra Info
Oxalac­etate
Acetyl-CoA + H2O
BLANK
( 1 )
Citrate synthase
Citrate
 
HS-CoA + H+
2
Aconitase
Isocitrate
NAD+
( 3 )
Isocitrate dehydr­ogenase
alpha-­ket­ogl­utarate
HS-CoA + NAD+
NADH + CO2
( 4 )
Alpha-­ket­ogl­utarate dehydr­ogenase
Succin­yl-­Coe­nzyme A
ADP + Pi
CO2 + NADH
5
Succinyl coenzyme A synthetase
Succinate
Ubiquinone (Q)
ATP + HS-CoA
6
Succinate dehydr­ogenase
Fumarate
H2O
QH2
7
Fumarase
Malate
NAD+
8
Malate dehydr­ogenase
Oxalac­etate
BLANK
NADH + H+

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